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KMID : 1149220110190020109
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Korean Journal of Oriental Medical Prescription
2011 Volume.19 No. 2 p.109 ~ p.118
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Research for the antiinflamation and antioxidation effect on the Lycoris squamigera Maxim
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Kim Bo-Mi
Yoo Myung-Ja
Song Mi-Seon
Kwon Tae-Oh
Lee Young-Hang
Chai Kyu-Yun
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Abstract
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Objectives: The Lycoris squamigera Maxim has been used traditionally for treatment of various diseases. However, the studies on the effect of Lycoris squamigera Maxim have not been carried out. In the present study, extract of Lycoris squamigera Maxim were tested for their anti-inflammatory and anti-oxidation effect.
Methods: The anti-inflammatory effect of the various solvent extract was studied in lipopolysaccharide (lps)-treated mouse macrophage cells. RAW 264.7 cells were pre-incubated with Lycoris squamigera Maxim extracts for 4h and treated with lps for 18h, and then the anti-inflammatory effects of extracts were determined. The anti-oxidation effect of extracts measured by DPPH method, reductive potential test, total phenolics test.
Results: Extracted root’s ethyl acetate layer showed a significant decrease in nitric oxide. And that layer (root’s ethyl acetate extract) was showed decrease in TNF- concentration dependently. Separated from Root’s ethyl acetate extract was fraction 1 has range, fraction 2 has range did not showed cytotoxicity. Anti-oxidation result as DPPH test showed the best was root ethyl acetate extract. Redusing power was made a comparison between fractions and standard. They were showed similar value. Fraction’s total phenol containing result was better then standard.
Conclusions: These results suggest that these extracts can be used as anti-inflammatory, anti-axidation materials.
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KEYWORD
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Lycoris squamigera Maxim, nitric oxide, TNF-, nitric oxide synthase, anti-inflammatory effect, anti-oxidation effect
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